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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
21/02/2014 |
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Actualizado : |
13/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
NOYES, N.R.; WEINROTH, M.E.; PARKER, J.K.; DEAN, C.J.; LAKIN, S.M.; RAYMOND, R.A.; ROVIRA, P.J.; DOSTER, E.; ABDO, Z.; MARTIN, J.N.; JONES, K.L.; RUIZ, J.; BOUCHER, C.A.; BELK, K.E.; MORLEY, P.S. |
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Afiliación : |
NOELLE R. NOYES; MAGGIE E. WEINROTH; JENNIFER K. PARKER; CHRIS J. DEAN; STEVEN M. LAKIN; ROBERT A. RAYMOND; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE DOSTER; ZAID ABDO; JENNIFER N. MARTIN; KENNETH L. JONES; JAIME RUIZ; CHRISTINA A. BOUCHER; KEITH E. BELK; PAUL S. MORLEY. |
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Título : |
Enrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Microbiome, 2017, 5, p. 142 |
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Páginas : |
13 p. |
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DOI : |
10.1186/s40168-017-0361-8 |
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Idioma : |
Inglés |
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Notas : |
Article History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017 |
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Contenido : |
Background: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins.
Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of
microbiome-resistome dynamics. MenosBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistan... Presentar Todo |
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Palabras claves : |
ANTIMICROBIAL RESISTANCE; METAGENÓMICA; MICROBIAL ECOLOGY; MOLECULAR ENRICHMENT; RARE MICROBIOME; RESISTOME. |
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Thesagro : |
ANALISIS BIOLOGICO; ECOLOGIA MICROBIANA; RESISTENCIA A AGENTES DANINOS. |
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Asunto categoría : |
U30 Métodos de investigación |
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Marc : |
LEADER 03225naa a2200433 a 4500
001 1032862
005 2018-09-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s40168-017-0361-8$2DOI
100 1 $aNOYES, N.R.
245 $aEnrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing.$h[electronic resource]
260 $c2017
300 $a13 p.
500 $aArticle History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017
520 $aBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias. Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins. Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of microbiome-resistome dynamics.
650 $aANALISIS BIOLOGICO
650 $aECOLOGIA MICROBIANA
650 $aRESISTENCIA A AGENTES DANINOS
653 $aANTIMICROBIAL RESISTANCE
653 $aMETAGENÓMICA
653 $aMICROBIAL ECOLOGY
653 $aMOLECULAR ENRICHMENT
653 $aRARE MICROBIOME
653 $aRESISTOME
700 1 $aWEINROTH, M.E.
700 1 $aPARKER, J.K.
700 1 $aDEAN, C.J.
700 1 $aLAKIN, S.M.
700 1 $aRAYMOND, R.A.
700 1 $aROVIRA, P.J.
700 1 $aDOSTER, E.
700 1 $aABDO, Z.
700 1 $aMARTIN, J.N.
700 1 $aJONES, K.L.
700 1 $aRUIZ, J.
700 1 $aBOUCHER, C.A.
700 1 $aBELK, K.E.
700 1 $aMORLEY, P.S.
773 $tMicrobiome, 2017, 5, p. 142
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
18/05/2023 |
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Actualizado : |
18/05/2023 |
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Tipo de producción científica : |
Abstracts/Resúmenes |
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Autor : |
FEDERICI, M.; RIGAMONTI, N.; FERRARI, G.; ROVIRA, P.J.; TORRES, P.; GUERRA, S.; SIMÓN, C.; FERNÁNDEZ-CIGANDA, S. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA RIGAMONTI, Departamento de Aguas y Evaluación del Impacto Ambiental. Laboratorios Tecnológicos del Uruguay-LATU; GRACIELA FERRARI, Departamento de Aguas y Evaluación del Impacto Ambiental. Laboratorios Tecnológicos del Uruguay-LATU; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ANDRES TORRES ASTETE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA GUERRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Departamento de Aguas y Evaluación del Impacto Ambiental. Laboratorios Tecnológicos del Uruguay-LATU; CLAUDIA SIMÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Análisis de comunidades de cianobacterias y microorganismos asociados al sedimento mediante secuenciación del gen 16S ARNr y qPCR en el embalse rincón del bonete. 161. (resúmen) |
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Complemento del título : |
Áreas temáticas: Ciencias del Mar y Limnología. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.145. |
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ISSN : |
1669-5410 |
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Idioma : |
Español |
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Notas : |
Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable". |
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Contenido : |
Las cianobacterias pueden producir cianotoxinas altamente tóxicas para la salud humana y animal. Si bien éstas se asocian al período estival, en los últimos años en Uruguay se han registrado durante todo el año, especialmente en cuerpos de agua lénticos como lagunas, tajamares y embalses. |
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Palabras claves : |
Cianobacterias; Gen 16S ARNr; QPCR; Secuenciación. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17149/1/FedericiMT-et.al-p145-3er-Congreso-Nacional-Biociencias-2022.pdf
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Marc : |
LEADER 01380nam a2200265 a 4500
001 1064133
005 2023-05-18
008 2022 bl uuuu u01u1 u #d
022 $a1669-5410
100 1 $aFEDERICI, M.
245 $aAnálisis de comunidades de cianobacterias y microorganismos asociados al sedimento mediante secuenciación del gen 16S ARNr y qPCR en el embalse rincón del bonete. 161. (resúmen)$h[electronic resource]
260 $aIn: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.145.$c2022
500 $aResumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".
520 $aLas cianobacterias pueden producir cianotoxinas altamente tóxicas para la salud humana y animal. Si bien éstas se asocian al período estival, en los últimos años en Uruguay se han registrado durante todo el año, especialmente en cuerpos de agua lénticos como lagunas, tajamares y embalses.
653 $aCianobacterias
653 $aGen 16S ARNr
653 $aQPCR
653 $aSecuenciación
700 1 $aRIGAMONTI, N.
700 1 $aFERRARI, G.
700 1 $aROVIRA, P.J.
700 1 $aTORRES, P.
700 1 $aGUERRA, S.
700 1 $aSIMÓN, C.
700 1 $aFERNÁNDEZ-CIGANDA, S.
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